Barcelona coach Gerardo Martino could not explain how his side collapsed in the second half of their 3-2 home loss to Valencia on Saturday.
The defeat ended a run of 25 straight league victories at Camp Nou, stretching back to round seven of the 2012-13 season when they were held to a 2-2 draw by Real Madrid.
The reigning Spanish champions went into the half-time break at 1-1 after Daniel Parejo had equalised Alexis Sanchez’ opener but second-half goals from Pablo Piatti and Paco Alcacer - either side of a Lionel Messi strike - gave los Che a famous victory.
"After the first 30 minutes, I can’t explain how the game changed after that. We were a different team in the second half,” Martino told a press conference.
"Before the game I said that one pays dearly for every mistake from now on and we made several today.
"When you have the chance to finish off your opponent, you must do it. You shouldn't give them chances to come back. Today we did that and Valencia took advantage."
The defeat means second-placed Atletico Madrid will have the chance to go three points clear at La Liga’s summit when they host Real Sociedad on Sunday, while Real Madrid can leapfrog Barca with a win at Athletic Bilbao.