Vicente Del Bosque has made one surprise change from the triumphant quarter-final line-up as the Sevilla striker makes his first start of the tournament for La Roja
The Spaniards have never lost a semi-final in a European Championship and Vicente Del Bosque will hope to maintain that record as he makes one change to the side that defeated France 2-0 in the previous round. In a surprising twist, Fabregas will begin the game on the bench as Negredo leads the line ahead of Fernando Torres and Fernando Llorente, despite only playing one minute of normal time in the tournament so far.
At the other end of the pitch La Roja have not conceded in 299 minutes, since Antonio Di Natale equalised for Italy in their opening game of the tournament. Another clean sheet could help see goalkeeper Iker Casillas become the first ever player to earn 100 international victories.
An injury to striker Helder Postiga picked up in the quarter-final against Czech Republic means that Paulo Bento is unable to field the same starting line-up for the seventh consecutive competitive fixture. Hugo Almeida replaces him in the only change for the Seleccao, with young Benfica forward Nelson Oliveira among the substitutes.
Almeida will be flanked by Cristiano Ronaldo who has scored three goals in his last two games. The Real Madrid forward will overtake Rui Costa to become the nation's third-highest capped player with his 95th appearance. Pepe and Fabio Coentrao are both declared fit to start despite sitting out training on Saturday to rest their injuries.
Portugal (4-3-3): Patricio; Pereira, Pepe, Bruno Alves, Coentrao; Moutinho, Veloso, Meireles; Nani, Almeida, Ronaldo.
Subs: Beto, Eduardo, Costa, Rolando, Lopes, Custodio, Micael, Viana, Quaresma, Oliveira, Varela, Postiga.
Spain (4-3-3): Casillas; Arebloa, Ramos, Pique, Alba; Alonso, Busquets, Xavi; Silva, Negredo, Iniesta.
Subs: Reina, Valdes, Albiol, Martinez, Juanfran, Cazorla, Navas, Mata, Pedro, Llorente, Torres, Fabregas.