Reports had suggested that a request from the RFEF to commemorate the Real Betis defender had been rejected, but the football governing body have dismissed those claims
The Real Betis defender lost his battle with pelvic cancer earlier this week, aged 23, and La Roja stars Cesc Fabregas and Sergio Ramos led the tributes to the former Under-19 international.
Spain's players had hoped that Roque's passing would be formally recognised just before kick-off in their meeting with Paulo Bento's men in Donetsk, but the European football governing body has denied having received any such request.
A statement on Uefa's website read: “Further to media reports of the alleged refusal to allow a minute of silence in memory of Spanish football player Miki Roque ahead of tonight's Uefa Euro 2012 semi-final between Spain and Portugal, Uefa would like to underline that it has not received any such request from the Spanish Football Federation (RFEF).
“Articles reporting its rejection by Uefa are therefore totally unfounded.”
Roque, who spent four years at Liverpool, was diagnosed with cancer last year after undergoing a routine check-up on a back problem and had a tumour removed last May.
Uefa allowed a minute's silence for French journalist Thierry Roland ahead of France's clash with Sweden during the group stages.