The Czechs must win the match to guarantee a place in the quarter-finals of Euro 2012 and are currently high in confidence after their 2-1 win over Greece on Tuesday.
However, they could be without their captain, who is struggling with a calf injury, and coach Michal Bilek admitted his fitness is still in the balance.
|Michal Bilek is constantly backing his underfire striker Milan Baros, however, it's his captain Tomas Rosicky who remains instrumental to his side's chances of progressing to the latter stages.
The 31-year-old playmaker limped off at half-time against Greece, yet he left his mark on a first half performance that was the best under Bilek's tenure.
"I played 20 minutes with just the use of one leg," said Rosicky after that win. Imagine the damage he can inflict on the co-hosts if he is passed fit on Saturday.
"It was his Achilles heel but we hope he is fit for the third match because he is our key player."
Bilek went on to praise his side for their victory over the Greeks.
"There is not one player on the team I would not praise," he added,
"We wanted to attack even more but it was difficult when Rosicky left."
The Czech Republic brought in Tomas Hubschman and moved Petr Jiracek to the right wing to great effect against Fernando Santos' men.
And, referring to his tactics for the game, Bilek continued: "In this match we wanted to close the defence.
"That is why Jiracek played in a position that is not usual for him. He performed very well and was making runs along the line and really getting in to the game."