The World Cup-winning boss did not reveal his starting XI for Friday's game in Prague, but says there will be a chance for everyone over the two matches
The World Cup winners have already cleared the qualification barrier for Poland and Ukraine but will be eager to maintain their perfect run in Group I.
Del Bosque called for determination and professionalism from his players and urged them to keep their foot on the pedal as they hope to finish with eight wins from eight.
"We respect the Czech Republic as they made us work in the last game against them. They have fast midfielders with good technique," the Spanish coach explained at the pre-match press conference in Prague.
And any suggestion of taking it easy with their place at Euro 2012 already booked was rejected by the Salamanca-born trainer.
"We have to be good professionals, good sportsmen. The worst thing that could happen would be for us to play without any belief or emotion," he said.
There have been suggestions that Fernando Torres could get the nod in Prague on Friday night, but the coach made it clear that everyone would be given a chance to play as he kept his cards close to his chest.
"We would love everyone to play in the starting XI because they are all excellent players," he added.
"But there are two games. so I hope everyone in the squad will be able to play at some point."
Kick-off against the Czech Republic is at 2045 (CET) in Prague.