The Atletico boss failed to control his anger at what he perceived was a lack of respect from Raphael Varane towards the end of the Champions League final
His side's hopes of landing the trophy for the first time were dashed in the cruellest of circumstances as they led 1-0 until deep into injury time when Sergio Ramos headed home a dramatic equaliser.
Atletico's exertions in winning the Liga title last weekend then caught up with them as Gareth Bale, Marcelo and Cristiano Ronaldo, from the penalty spot, all scored in extra-time as Real Madrid finally got their hands on La Decima.
In the aftermath of Ronaldo's penalty, Varane kicked the ball towards the Atletico dug-out, prompting the Argentine to storm the field in an attempt to confront the young Frenchman.
Simeone's discontent had been simmering for some time although previously his anger had been reserved for referee Bjorn Kuipers for the amount of time he had allowed at the end of the 90 minutes.
Players from both sides, as well as Atletico staff members, had to hold Simeone back before he was ultimately ushered back to the technical area.
Atletico skipper Gabi refused to criticise his boss, although he did admit: "You have to know how to win and he is still young. He did an ugly gesture."
Simeone's anger, meanwhile, had cooled by the time he addressed the media: "Hopefully this will provide Varane with an experience and he will learn. He's a great player with a great future."