Pep Guardiola recalls the two midfielders, but is without both Xavi and Pique for the last-16 clash in Germany. Sergi Roberto, Isaac Cuenca and Marc Bartra are all on the bench
All three midfielders were left on the bench as a weakened Barca team lost 3-2 to Osasuna in La Liga on Saturday, but Xavi is still feeling the effects of an injury and misses out altogether.
Gerard Pique is also out as Javier Mascherano starts in defence, but Sergio Busquets is back in midfield.
Leverkusen, meanwhile, start with no recognised striker as Andre Schurrle leads the line for the Bundesliga outfit.
On-loan Tottenham full-back Vedran Corluka also features for the home side.
Leverkusen (4-3-2-1): Leno; Corluka, Schwaab, Friedrich, Kadlec; Rolfes, Reinartz, Bender; Renato Augusto, Castro; Schurrle
Subs: Yelldell, Oczipka, Da Costa, Omer Toprak, Ortega, Bellarabi, Kiessling
Barcelona (4-3-3): Valdes, Alves, Puyol, Mascherano, Abidal; Fabregas, Iniesta, Busquets; Adriano, Messi, Alexis
Subs: Pinto, Bartra, Thiago, Dos Santos, Sergi Roberto, Pedro, Cuenca