Gerard Pique and Carles Puyol partner each other at the back as Xavi, Andres Iniesta, and Javier Mascherano are left on the bench for the European champions
The Catalan side managed to defeat the Czechs 2-0 in their last European match, and Pep Guardiola also awards Isaac Cuenca another start as the young Spaniard continues to impress in attack.
There is no place in the squad for David Villa as Adriano takes up the role on the left of the attacking trio, although Cesc Fabregas is likely to push forward into the attacking positions.
Viktoria Plzen opt for a five man midfield against the European champions and Marek Bakos is selected in the lone striker role.
Viktoria Plzen (4-5-1): Pavlik; Limbersky, Bystron, Cisovsky, Pilar; Horvath, Petrzela, Jiracek, Kolar, Rajtoral; Bakos
Subs: Tichacek, Reznik, Sevinsky, Fillo, Darida, Duris, Hora
Barcelona (3-4-3): Valdes; Pique, Puyol, Abidal; Alves, Fabregas, Thiago, Busquets; Adriano, Cuenca, Messi
Subs: Pinto, Maxwell, Xavi, Iniesta, Mascherano, Keita, Sanchez