Should tonight's Clasico turn sour, Del Bosque is vehement there will be no repercussions on the Spain national team
Both sides have been locked in bitter disputes since the Catalan club emerged 2-0 winners in the first-leg - a game marred by controversy on and off the pitch.
"I appreciate the concern showed by fans and media that the mood in the last few games could lead to possible collateral damage that could affect the relationship between players of both sides within the Spanish football team," Del Bosque told AS.
"They are used to this kind of game and this rivalry is not new. It is my hope that [the conflict and tension] does not pass over or move to the [national team]."
Del Bosque was also keen to stress that any potential clashes between the two sets of teams in tonight's tie would not affect his squad selection for the World Cup champions.
"Players must be consistent with their actions and they alone are responsible for winning or losing the privilege of belonging to the squad of the Spanish national team.
Finally, the Champions League winning coach expressed his desire to see the beautiful game take centre stage in what is the fourth Clasico in the past 18 days.
"The key word is respect... Respect and winning spirit as a team. All for one."