The Bianconeri defender expressed his defiance ahead of his side's trip to Istanbul, while Arturo Vidal says they will embrace their role as "villains"
The Old Lady travel to the Turk Telekom Arena for their final Champions League Group B game having amassed six points from their five games - two ahead of their hosts - and needing only to avoid defeat to progress.
However, though the Juventus players are bracing themselves for a hostile reception, Chiellini insisted that the team are unflustered.
"In Istanbul it will be like playing a final," the 29-year-old wrote on his official Facebook page.
"We're not afraid, we are Juve."
Galatasaray fans have been known to go to great lengths to make visiting teams feel uncomfortable, camping outside hotels to disturb rival players' sleep with horns and creating a raucous atmosphere from the moment they arrive.
However, Arturo Vidal also echoed Chiellini's assertion that the Bianconeri would not be worried by the response of the home support.
"We will take to the field as villains," the Chilean told reporters ahead of the game.
"We need to start strong need to understand who we are. If we play with our usual competitiveness it's difficult for anyone to beat us."
Juventus go into Tuesday's clash after securing five straight wins in all competitions, while Gala warmed up for the encounter with a 2-0 win over Elazigspor on Friday.