Barca started their league campaign with a 3-0 home win over Elche last week and will welcome back Brazil star Neymar for the Villarreal clash.
And although Andres Iniesta (knee) will miss the match, Luis Enrique is confident and hopes to see his team gel.
"I am hoping that the team begins to settle which is something that will happen with games," he said.
"We are looking sharp after a week where we have trained well. We know our rival well and they are a strong team in the league.
"They had a good pre-season in terms of performance and results.
"The game will not be easy for us but we are prepared and, at the moment, each match is a test for us. All teams with a new coach need time to adapt but that is something that I am not asking for."
The 44-year-old also confirmed that new signing Douglas can expect to make his debut for the club in the near future.
"He’ll be good for us and I thank the club for it’s hard work and effort to bring him here," Luis Enrique said.
"In principle, I’ll be playing him in his natural position, but there’s always the option of moving him further up the field.”