The Portugal international has been beset by muscular problems since the tail end of last season but the Che boss fully expects the Ballon d'Or holder to feature in Cardiff
The Portugal international played a part in Blancos' pre-season training for their International Champions Cup endeavours in USA but did not feature in the tournament.
Ancelotti claims that Ronaldo will be part of the starting line-up in the clash between the Champions League winners and Europa League victors but Madrid remain worried by his chronic knee problems.
However, Emery is expecting Los Blancos to field their strongest possible line-up in Cardiff.
"I do not think about who will play for Madrid," he told Marca when asked if it would be better for Sevilla if the 2014 Goal 50 winner Ronaldo did not play. "I'm sure they will play with their best 11 players.
"If Cristiano Ronaldo is fit, he will play. Toni Kroos, though he's had less training, may play. We are working on each of their best players and how to stop them, regardless of rumours."
Although Sevilla head into Tuesday's game as underdogs, Emery has every belief that they can spring a surprise against their fellow Spaniards.
"The Supercopa is a prize which we deserve a chance at winning," he argued. "We're heading into the game thinking only about winning and we have conviction that we can do it.
"The difficulties of beating Madrid are ones we have to face. But we also want to enjoy this occasion with all of Sevilla's fans."
Kick-off between Real Madrid and Sevilla is at 20:45CET.