The centre-back, 35, underwent surgery in June to remove a cyst from his right knee and had been absent for Gerardo Martino's men until the 0-0 draw with Osasuna in Pamplona.
It was the first time Barca have dropped points in La Liga this season, but the defender wants his team-mates to put the draw behind them and focus their energy on Tuesday's Champions League draw with AC Milan.
"These past few months have been difficult for me," Puyol told reporters.
"I am happy with how I felt on the pitch but not with our result. Now we must rest, recover and start thinking about AC Milan."
Barcelona sit at the summit of the Liga table, with 25 points amassed from a possible 27 thus far.
Gerardo Martino's men head into their Champions League fixture with Milan having won both of their opening Group H fixtures against Ajax and Celtic respectively.