The former Espanyol forward is handed a chance in a 4-2-3-1 formation as Jose Mourinho's men look to return to winning ways in La Liga following last weekend's loss to Barcelona
Callejon impressed as he opened the scoring in Madrid's 2-0 win over Ponferradina in the Copa del Rey on Tuesday night, and the former Espanyol man has been handed a rare league start by coach Jose Mourinho in a game Real will be eager to win to move three points clear of Barca at the top of the table.
Elsewhere, Alvaro Arbeloa returns at right-back after he was left out in favour of Fabio Coentrao against the Catalans, and Benzema begins up front as Gonzalo Higuain is on the bench along with Kaka and Mesut Ozil.
Sevilla start with an attack-minded 4-3-3 formation, with Jesus Navas, Manu del Moral and Alvaro Negredo leading the line and Frederic Kanoute on the bench.
Sevilla (4-3-3): Javi Varas; Coke, Caceres, Spahic, Navarro; Medel, Trochowski, Rakitic; Navas, Manu del Moral, Negredo
Substitutes: Sergio, Luna, Escude, Armenteros, Coke, Kanoute, Campana
Real Madrid (4-2-3-1): Casillas, Arbeloa, Pepe, Ramos, Marcelo; Xabi Alonso, Diarra; Callejon, Di Maria, Ronaldo; Benzema.
Substitutes: Adan, Albiol, Khedira, Altintop, Kaka, Ozil, Higuain