A cacophony of boos greeted Pablo Osvaldo on to the pitch when Antonio Conte sent him on with the tie looking destined for a 0-0 stalemate. Perhaps motivated by the negative reception from the Stadio Olimpico faithful he ditched for Southampton last summer, he gladly accepted the role of villain by scoring the winner with practically the last kick of the game.
Grinning with masochistic delight as the loanee walked on to the pitch he spent two years playing at regularly, Osvaldo did not see much of the ball but deep in stoppage-time ruined Roma's final home game of the season by striking smoothly into the top corner from inside the box.
As they watched their former hero hold his hand up his ear, Roma fans must have wondered how differently their season could have ended had they kept up the stunning start to 2013-14 they unexpectedly showed.
Their incredible winning streak - 10 wins in a row from the opening game - saw them sit at the top of the league for a long while, threatening to usurp Juve as kings of Italy.
But draws became commonplace for the Giallorossi, while Juve were typically ruthless, winning every single one of their home games and only dropping points in five Serie A matches heading into the Stadio Olimpico clash.
Vasilis Torosidis put the ball in the back of the net but it was correctly disallowed as the defender had clearly scored (albeit accidentally) with his hand, Gervinho fluffed a one-on-one chance as half-time lingered and substitute Alessandro Florenzi was unfortunate to be denied a rebound goal late on.
But, thanks to Osvaldo's first Serie A goal of the season, Juve provided a timely reminder that it is small margins which ultimately decide title winners. Roma simply have not shown that killer instinct which the Scudetto holders have in abundance.
Gervinho has shed his Arsenal skin and shown why the Gunners splashed out for him in the first place by shining under his former Lille coach Garcia in a Giallorossi shirt.
Marshalled in midfield by Daniele de Rossi, the capital club definitely could fight for the title until the last day next year if they bolster up their squad in the summer, patch up their weak spots and learn to finish off teams.
But this Serie A season has once again belonged to Juventus as they sit one win or draw away from becoming the first Italian team to break the 100-point barrier.
They might be their equals in the near future, but for now Roma will have to accept that they and everyone else in the league remain in Juve's shadow.