The Chilean did not return to Turin until late on Friday after missing his flight following his country's 2-1 victory over Ecuador that sealed their place in next year's World Cup finals.Vidal was subsequently unable to train on Friday ahead of the crucial clash with Fiorentina on Sunday, much to the frustration of Conte.
"I haven't spoken to Vidal yet," he told a press conference. "I need to understand why he was so late.
"He wasn't at training yesterday either.
"Will he be punished? The rules are the same for everyone."
Juventus' trip to Florence comes just days prior to a crucial Champions League clash with Real Madrid, but Conte made it clear he is not distracted by the visit to Santiago Bernabeu next Wednesday.
"Real Madrid do not interest me. I don't wish to be rude, but right now the only thing in our heads is Fiorentina," he said.
"The Viola are a great team with a wonderful project in place. This side was built to improve upon last season’s fourth-place finish, so they are not a surprise.
"Anyone who faces them is on the alert.
"If we want to do something extraordinary this year, then we'll have to fight to get it. If we are aware of that fact, then we can do it."
Roma moved five points clear of Juve with a 2-0 win over Napoli on Friday, and Conte is under no illusions as to the threat posed by Rudi Garcia's side in the title race.
"Roma-Napoli was a match between two candidates for the Scudetto," added the 44-year-old. "They will be in the race right to the end."