Play was halted during AC Milan's clash with the capital club after visiting supporters aimed abusive chants at the former Manchester City striker
The referee was forced to temporarily call a halt to proceedings just after half time following an announcement made via the tannoy stating the game would remain suspended until the abuse stopped.
Roma skipper Francesco Totti was asked by the official to use his influence with the supporters by speaking to a number of them in a bid to put an end to the singing, which appeared to prove effective.
Earlier this year, Balotelli's former club Inter were fined for their fans' treatment of the Italy international during the Milan derby in February.
Milan have been involved in several similar incidents this season, with Kevin-Prince Boateng leaving the pitch during a friendly against Pro Patria in January.
More recently, Juventus fans were fined after subjecting the former Ghana midfielder to racist chants.
Tempers flared throughout Milan's clash with Roma, which finished 0-0, with both Sulley Muntari and Totti being sent off.