The 49-year-old coach believes Antonio Conte's side are not unbeatable on the eve of facing the champions-elect but says he wants to see his players give "150 per cent"
Antonio Conte's men are coasting to title glory in Serie A for a second successive season with the Stadio Olimpico being the next stop en route to retaining their crown.
However, the Juventus coach is yet to experience victory over Lazio as a trainer and Petkovic says his team have every chance of slowing the inevitable by beating the Bianconeri.
"We will never give up. We have to try and prevent them from playing at their best," he said.
"They have pretty much already won the Scudetto, but football teaches us that there are no unbeatable teams. We will make Juventus sweat to get the result."
Like Juventus, Lazio were sent crashing out of European competition during the week, with Fenerbahce progressing to the semi-finals of the Europa League over Petkovic's men.
The 49-year-old went on to praise the performance of his players but insisted a super-human effort was required to prevent Juventus from moving nearer to retaining their title.
"The team are ready and I complimented them because they gave 100 per cent on Thursday. But with Juve, they have to give 150% because when playing against the best in the league so further effort is necessary," he added.
The Bosnian also revealed he was positive his side could catch AC Milan who currently occupy the third and final Champions League place but admits Lazio's fate lies in the hands of their rivals.
Petkovic said: "I'm optimistic, but it depends on the teams in front of us because they need to drop points while we have to keep winning."
Following the visit of Juventus, Lazio travel to Udinese next Sunday.