The Biancocelesti have been punished after Aaron Lennon and Jermain Defoe were abused by the Italian club's travelling support during the Group J encounter
The punishment relates to abuse directed at two of Spurs' players on the night, Jermain Defoe and Aaron Lennon.
The announcement, which was made on Uefa's website on Thursday, also stated that the Italian capital club have three days to lodge an appeal against the decision.
Both teams opened their group stage campaign in the match, which ultimately ended goalless, with both Clint Dempsey and Steven Caulker having goals disallowed.
Tottenham manager Andre Villas-Boas spoke to reporters about the alleged chanting after the Group J tie.
"Uefa is very active on this kind of situation, and our overall objective is to kick racism out of football," said the 35-year-old.
"I can't point the finger at anyone in any way, shape or form tonight, because I didn't hear anything," he added.