Police have assured the Olimpico will be safe enough for fans to enjoy a good night of football after violent incidents marred the build-up in the capital over the weekend
A number of incidents took place on Saturday as armed people, believed to be supporters of both teams, marauded around Rome's streets intent on causing trouble.
Police clashed with troublemakers and around 70 people were injured during a day of violence in the Italian capital.
Fears of the derby being called off spread as riots escalated, but Rome's mayor Gianni Alemanno said the game will proceed.
"The derby will be played, it would be a defeat for the city of Rome if it was cancelled," Alemanno is quoted as saying on Il Corriere dello Sport.
The president of the Lega Calcio (Italian Football League), Maurizio Beretta, is also sure that security measures in place will lock out any further trouble.
"I have spoken with the police and they assured me that the stadium and the surrounding areas have been made safe for the fans," said Beretta.
Kick-off at the Olimpico is at 20.45 (CET).