Gerardo Martino's side must win if they are to retain their title, as the visitors go in search of a first league crown since 1996
The visitors head into the weekend's showdown holding the advantage over their opponents, with a three-point lead over the reigning champions at the top of the table.
However, a win for Barca would see them pip Atletico to the title on the basis of their head-to-head record following a 0-0 draw at Vicente Calderon back in January.
That stalemate opened the door for Atletico to clinch their first crown since 1996, but they too were left frustrated, drawing 1-1 with Malaga last time out after they had lost 2-0 at Levante one week earlier.
Atleti striker Diego Costa is expected to be fit for the clash after a niggling hamstring problem, although Cristian Rodriguez is still struggling with a similar injury.
For Barca, Neymar (toe) and Jordi Alba (hamstring) have not featured since the Copa final and remain doubts, while Gerard Pique's fitness is also uncertain after he cracked his hip in the Champions League quarter-final first leg between the two sides.
However, all three took part in fitness training sessions this week with a view to being ready for the Camp Nou showdown this weekend.