Los Che's centre-back is set to miss next week's Champions League round of 16 tie in Paris after tearing a hamstring during the Liga match against Mallorca over the weekend
Valencia are looking to overcome a 2-1 deficit from the first leg at the Mestalla Stadium, which saw Rami’s late goal and Zlatan Ibrahimovic’s last-minute sending off give Los Che a chance in the tie.
The central defender is not expected to return to action until the home game against Real Betis, and will also miss the forthcoming league fixtures against Zaragoza, Levante and Athletic Bilbao.
Rami has been a prominent figure for his club this season, with 33 appearances in all competitions, including six in the Champions League.
Valencia currently lie fifth in La Liga with the fourth Champions League spot currently filled by Malaga.