Andre Villas-Boas names a strong team for the Europa League trip to Greece, making only three changes from the side that won 3-2 over Manchester United at Old Trafford on Saturday
Hugo Lloris takes the place of Brad Friedel in goal for his second Spurs start, whilst William Gallas and Sandro are replaced by Michael Dawson and Tom Huddlestone respectively.
The team has a similar look to the one that took on Lazio at White Hart Lane, as Villas-Boas seems set on taking the competition very seriously.
The former Chelsea boss won the Europa League whilst in charge of Porto and looks determined to bring the trophy to London this season.
Panathinaikos are also coached by a former Porto boss in Jesualdo Ferreira and include former Arsenal, Cardiff City and Birmingham City forward Quincy Owusu-Abeyie among their line-up.
Panathinaikos starting XI: Karnezis, Andre Pinto, Seitaridis, Marinos, Vyntra, Triantafyllopoulos, Vitolo, Christodoulopoulos, Zeca, Toche, Owusu-Abeyie
Subs: Kotsolis, Chouchoumis, Sissoko, Mavrias, Lagos, Fornaroli, Barbarouses
Tottenham starting XI: Lloris, Walker, Dawson, Caulker, Vertonghen, Huddlestone, Dembele, Dempsey, Lennon, Bale, Defoe
Subs: Cudicini, Gallas, Sandro, Townsend, Falque, Mason and Sigurdsson