The Clever Boys won the 2016/17 league title after Mamelodi Sundowns were held to a draw by Maritzburg United, while Rise and Shine remain sixth on the log.
Wits coach Gavin Hunt made four changes to the starting line-up that faced Free State Stars last week. Darren Keet, James Keene, Daine Klate and S'fiso Hlanti all made their return to the starting line-up.
The Clever Boys, who had suffered a 3-1 defeat to Stars at the Goble Park Stadium, knew that a victory over Polokwane would secure their first ever PSL title, if other results go their way on the night.
However, they faced a confident Polokwane side which had stunned Kaizer Chiefs 1-0 at the Peter Mokaba Stadium last weekend. Rise and Shine coach Bernard Molekwa had to do without suspended duo Sammy Seabi and Jabulani Maluleke.
The Clever Boys applied some pressure on the Rise and Shine defence from the start and they deservedly took an early lead through Keene's well-taken header just three minutes into the game.
The visitors were forced to try their luck from range as Wits captain Thulani Hlatshwayo and his troops looked solid at the back as they stopped the Rise and Shine attacks.
Lorence Vilakazi saw his effort from range being easily saved by Wits goalkeeper Keet as the Clever Boys defence continued to frustrate Rise and Shine.
Hunt's side increased the tempo after the half-hour mark and Hlanti played a great cross into the Polokwane box for Keene, whose header narrowly missed the target.
Wits should have been 2-0 up during the half-time break, but Elias Pelembe failed to hit the back of the net as his effort narrowly missed the target and Polokwane survived a scare.
The score was 1-0 to Wits during the half-time break following an action-packed first-half on a cold Wednesday night in Braamfontein.
The Clever Boys continued their dominance in terms of ball possession in the early stages of the second-half and Mogakolodi Ngele forced Chigova into a decent save.
However, the home side kept piling the pressure on the Rise and Shine defence and they did double their lead on the hour-mark when Ngele set-up Klate, who netted to make it 2-0.
Hunt then pulled out Botswana international Ngele towards the end of the match and he was replaced by defensive midfielder Phumlani Ntshangase as the home side looked to protect their healthy lead.
The former South Africa Under-23 international did well to stop the Polokwane attacks in the midfield and he also made key passes in the final third.
Former Kaizer Chiefs trialist Keene should have extended his side's lead in the closing stages of the game, but he was brilliantly denied by Chigova and ultmately, Wits secured a 2-0 victory.