The Brazil star has been out of action since suffering a back injury in his nation's World Cup quarterfinal victory against Colombia, but he is fit and ready to return.
Neymar has been given the all clear to make his Barcelona comeback in the club's friendly with Leon on Monday.
The Brazil international has not featured in Barca's preseason schedule so far after suffering a back injury during his nation's 2-1 quarterfinal win over Colombia at the World Cup last month.
The 22-year-old forward returned to training last week, however, and he could now feature at Camp Nou against the Mexican side when Barca battles for the Joan Gamper Trophy.
In a further boost, the club also confirmed that experienced midfielder Xavi is free to feature after he suffered a calf strain in training at the beginning of August.
"The first team squad trained this morning ahead of the Joan Gamper Trophy at the Camp Nou against Club Leon," read a statement on Barca's website. "At the end of the session, club doctors gave Neymar and Xavi Hernandez the medical all clear and both will be available for the game."
The fixture marks Barca's final preseason match before its Liga opener at home to Elche on Sunday.