The RFEF has turned down the Portugal international's appeal against his ban, meaning he will miss the games against Villarreal, Getafe and Elche.
Cristiano Ronaldo's appeal against his three-game suspension following his dismissal in Real Madrid's 1-1 La Liga draw against Athletic Bilbao at the weekend has been rejected.
The prolific attacker was shown a straight red card after an altercation with Carlos Gurpegui and Ander Iturraspe and was consequently handed a three-match ban by the Spanish football association (RFEF) - one for the incident itself and another two for his gesture after he was sent off.
The Blancos disagreed with the severity of the suspension and therefore opted to file an appeal, but the Appeal Committee has now rejected the Santiago Bernabeu's plea.
Ronaldo will miss this Saturday's home game against Villarreal as a result, while he will also have to watch the matches against Getafe (Feb. 16, away) and Elche (Feb. 23, home) from the stands due to his ban.
The 29-year-old has been in sublime form for Madrid this season, netting 22 goals in 21 La Liga appearances.
The absence of Ronaldo means Ancelotti is likely to field Jese Rodriguez, Karim Benzema and Gareth Bale as his three-man attack until the Portuguese's return.