The Atletico Madrid forward has already been capped twice by the Selecao at the international level but he is eligible to play for La Roja as both games were friendlies.
The Atletico Madrid forward has already been capped twice by the land of his birth but both games were friendlies, meaning the 25-year-old, who secured Spanish citizenship during the summer, is free to represent La Roja.
Brazil had called Costa up for its own November internationals, against Honduras and Chile, but he was promptly dropped from the panel after formally confirming his decision to play for Spain.
The attacker has been joined in del Bosque's panel by Atletico teammate David Villa. Manchester City's in-form forward, Alvaro Negredo, has made the cut but there is no room for Tottenham striker Roberto Soldado.
Elsewhere, Iker Casillas is once again called upon, even though he remains second-choice to Diego Lopez at Real Madrid for Liga fixtures.
Goalkeepers: Iker Casillas (Real Madrid), Victor Valdes (Barcelona), Pepe Reina (Napoli).
Defenders: Alvaro Arbeloa (Real Madrid), Raul Albiol (Napoli), Sergio Ramos (Real Madrid), Alberto Moreno (Sevilla), Juanfran (Levante), Javi Martinez (Bayern Munich), Inigo Martinez (Real Sociedad), Nacho Monreal (Arsenal).
Midfielders: Sergio Busquets (Barcelona), Xabi Alonso (Real Madrid), Koke (Atletico Madrid), Andres Iniesta (Barcelona), Santi Cazorla (Arsenal), Cesc Fabregas (Barcelona), Jesus Navas (Manchester City).
Forwards: Alvaro Negredo (Manchester City), Diego Costa (Atletico Madrid), Pedro (Barcelona), David Villa (Atletico Madrid), Juan Mata (Chelsea).