Reports had circulated across Spain that the Portuguese star would not feature for Los Blancos against their city rivals, and assistant coach Aitor Karanka verified his absence.
"Cristiano Ronaldo will not be in the squad for tomorrow," Karanka stated at a press conference on Friday. "With the exception of Cristiano Ronaldo, Marcelo and [Alvaro] Arbeloa, the rest are well and tomrorrow the best team will go out to win."
Karanka went on to say that each member of the Blancos squad must give his all against Diego Simeone's side as the club looks to warm up for its Champions League clash with Borussia Dortmund on Tuesday in hopes of overturning a 4-1 deficit after the first leg.
"Tomorrow we have to be at 120 percent and go out with the maximum level of intensity," Karanka said. "There are still big games left and the team is going to be at that level. We have to go out tomorrow with a winning mentality and increase the points lead [over Atleti].
"The best way to present yourself for Tuesday at the Bernabeu is winning. It's the best way to get motivated."