The Argentina international suffered a hamstring injury in the first leg of the Champions League quarterfinal tie against Paris Saint-Germain and consequently started the return leg from the bench as he had not fully recovered.
Messi was brought on in the 62nd minute, though, as Barcelona was trailing 1-0 and facing elimination. The Blaugrana managed to restore parity after the prolific attacker's entrance, with Messi playing a major role in the build-up to Pedro's goal, thus securing qualification for the semifinals.
There were fears that the forward's substitute cameo had worsened his injury, but a series of tests have made it clear that is not the case.
"Tests have confirmed that Wednesday's appearance has not aggravated the injury. The player will continue his recovery process and his progress will mark his availability for the next few games," an official Barcelona statement reads.
Messi is likely to be rested for this weekend's La Liga match against Zaragoza.