The 44-year-old recently returned to the Blaugrana bench after undergoing surgery on a tumor back in December, but his place will be taken by his number two again for this week's cup match.
"Tito will not attend Thursday's encounter at Camp Nou. He had to leave for a series of medical tests and will not be available," he said at a press conference.
The Barcelona caretaker boss then went on to praise David Villa, saying that the experienced striker no longer needs to prove himself as he has already proven his worth over the years.
"I wouldn't say that the Copa del Rey match is a chance for Villa to prove himself. He does not need to prove himself," he continued.
"We all know that he is an extraordinary player. He always works hard and is still very important for Barcelona."
Barcelona won the first leg of the round of 16 match against Cordoba 2-0.