The Bavarians' 53-match unbeaten run in the Bundesliga was ended by Augsburg but their coach is keen to move on quickly.
A first-half Sacha Molders goal at the SGL Arena consigned the German champion to its first league defeat since it lost 2-1 at home to Bayer Leverkusen back in October 2012.
However, Guardiola has insisted that his team was beaten by a strong opponent and is looking forward to putting things right against Manchester United in the Champions League in midweek.
"No complaints. We lost because the opponent was a bit better than us," he told reporters. "We have already seen how good a team Augsburg are in the DFB Pokal. We fought and tried a lot. This is football. You can't always win. Sooner or later, a defeat happens.
"We accept that and now we need to prepare for the Manchester United game as soon as possible. On Wednesday it's a final. It's about life or death."
Forward Thomas Muller admitted that his teammates were disappointed that the long run without a defeat had come to an end.
"It hurts a bit because we wanted to keep our run going. The fact is that it's not easy in Augsburg. That was clear before the game, but the plan was not to go home in defeat."