The 77-year-old says he has no intention of selling the Rossoneri, despite reports this week to the contrary.
The former Prime Minister of Italy has owned the Rossoneri since the 1980s, overseeing a period of success both within Serie A and in Europe.
However, rumors circulated earlier this week that he was weighing up a sale, only for Fininvest, the club's holding company, to staunchly deny the claims.
And Berlusconi has now dismissed the reports once more, telling reporters in Rome: "I don't want to sell AC Milan, I can reassure Milan fans."
Milan is enduring a turbulent season on and off the pitch, with Berlusconi's daughter Barbara, a longtime board member, stepping into a more senior role earlier in the campaign.