David Luiz is back in training, while Mauricio Pochettino will hope to have Luke Shaw and Dejan Lovren available.
Mohamed Salah's late strike ensured the Swiss giant completed a famous double over the Blues, who have now won just two of their last five matches in all competitions.
Samuel Eto'o dealt boss Jose Mourinho a further blow by limping off during the defeat and will not face the Saints, with fit-again Fernando Torres expected to lead the line.
David Luiz is also back in training for the Blues after a knee injury but will not be risked on Sunday.
For Southampton, a trip to Stamford Bridge represents another test of the top-four credentials which took a dent in defeat to Arsenal last weekend.
Artur Boruc in particular will be seeking redemption after his clanger allowed Olivier Giroud the easiest of goals to give the in-form Gunners a lead they never relinquished on 22 minutes.
Despite the result, Mauricio Pochettino's side has made a superb start to the season, with just two losses from its opening 12 matches leaving the club in fifth place.
The visiting team has injury concerns of its own, with starlet Luke Shaw forced off at halftime against the Gunners at the Emirates Stadium after sustaining a knock.
Pochettino will hope to welcome back defender Dejan Lovren, after he missed the Arsenal game with a sickness bug.
But the match is likely to come too soon for Jack Cork, who has a twisted ankle.