Steven Gerrard was ruled out of the fixture at Wembley due to a hip injury, while Lampard will be awarded a golden cap prior to kickoff against Chile in recognition of his 100th cap.
The Chelsea midfielder, who became an England centurion against Ukraine in September, will receive his award from his father, Frank Lampard Sr. and Sir Geoff Hurst.
"It's going to be very special, 100 caps have been a long time coming," Lampard told reporters. "I've seen gold caps get rightly given to Stevie [Gerrard] and Ashley [Cole], and I'm very proud. Having Sir Geoff there was an FA idea, but I'm very happy with it because I've had links to him all my life through my dad, and he's an England hero.
"I requested my dad be involved because there aren't many international fathers and sons. It's the sort of thing he normally shies away from but with a bit of pressing he's managed to get himself out there, and I'm sure he'll be a very proud dad when he does."
Kyle Walker will also miss the match against Chile but England manager Roy Hodgson is hopeful that the Tottenham right back, along with Gerrard, will be fit to face Germany on Tuesday.
Hodgson confirmed that Joe Hart will not start in goal against Jorge Sampaoli’s side, meaning either Celtic’s Fraser Forster or Norwich City’s John Ruddy will deputize.
Southampton striker Rickie Lambert has suffered a muscle injury in training and has been ruled out of the match against Chile and is also a doubt for the match against Joachim Low’s side.
Liverpool forward Daniel Sturridge remains a doubt for Friday’s fixture due to a foot injury.