The Portuguese is furious that he and his medical staff have been attacked for opting against replacing the French shot-stopper after he was knocked out at Goodison Park.
The goalkeeper was knocked out after a collision with striker Romelu Lukaku yet continued after receiving lengthy treatment on the pitch.
Spurs have subsequently come in for criticism, with brain injury charity Headway describing the decision as "irresponsible and cavalier", while Liverpool's former medical chief, Peter Brukner, told Goal that it was "unacceptable".
However, an unrepentant Villas-Boas insists he made the right call and is disappointed by the way in which his staff have been called into question over the incident.
"I stand absolutely by the decision I took and I stand by the decision that the medical department took following the checks that they made on the player," the Tottenham trainer told a press conference. "All the checks were according to the book. I have registered the fact that a couple of people have taken this opportunity to find the chance to get themselves publicized who have no experience in the pitch whatever in these type of situations.
"And I find it extremely disappointing that two people - a great doctor [club doctor Shabaaz Mughal] and a great physio [Geoff Scott], who saved the life of [Fabrice] Muamba - were heavily put into question by lots of incompetent people, with absolutely no experience on the pitch, no experience in the action, no experience in the moment," he continued. "That is extremely serious and disappointing."