The Belgium international picked up the injury after scoring the Blues' second goal in Sunday's 4-1 victory over Malaysia XI, and he will play no further part in the club's tour.
De Bruyne landed awkwardly after smashing in the second goal in the Blues' 4-1 win, and he was immediately substituted. The Belgian later joined his teammates on the bench for the second half, allaying fears of serious knee ligament damage, and it was expected he would remain with the rest of the squad.
After the match, boss Jose Mourinho praised De Bruyne's performance, and he appeared confident the midfielder's injury was not as bad as first feared.
"When he was on the floor and asking for a change everybody thought the worst, but the first assessment by the doctor is that [it is not] ligaments, and if it's something in the meniscus it has to be a very small injury, so we believe it is not an important injury, and hopefully it isn't, because the kid is a fantastic player," Mourinho told reporters. "A kid, but a man on the pitch. Hopefully it's nothing important and he will be ready soon."