The Tottenham coach revealed that he was upset with the Real Madrid manager's decision to publicize talks between both clubs over the prospective transfer of Gareth Bale.
It emerged on Wednesday that Spurs had opened talks with Madrid over the 114 million euro sale of the forward, but Villas-Boas admitted that he was perturbed by Ancelotti’s decision to make such talks public.
Asked if it was inappropriate after his club's 5-2 defeat to Monaco on Saturday -- a match that saw Bale omitted through injury -- Villas-Boas told reporters: "I think so, in my opinion.
"Carlo is a person I appreciate a lot and we have great respect for each other, but bearing in mind this situation they have decided to make it public.
"Normally in situations like this it is scrutinized by the Football Association with lots of care and attention. We have seen lots of people speak about a player that is not theirs and we are due some respect.
"These rumors of an imminent transfer are not true. The only thing we have communicated with Real Madrid is that he is our player and he is not for sale.
"Gareth is a guy I really like. We are talking with Real Madrid because there is respect between the two clubs following the transfer last season of Luka Modric.
"We are building a great team for next season. We envisage the future with Gareth. But we know that in football anything is possible."
Tottenham faces Espanyol on Aug. 10 in its final preseason fixture before kicking off the Premier League campaign at Crystal Palace eight days later.