Spurs are one of only two teams left in the competition to have won all five previous group-stage matches and could continue the good form against Anzhi Makhachkala.
Spurs have won all five of their games in Group K and the Portuguese has challenged his players to make it six when they face Anzhi Makhachkala on Thursday.
Salzburg is the only other side in this year's competition which could achieve that feat and Villas-Boas is keen to see his side extend its four-game unbeaten run in all competitions.
"Another win would be brilliant for us," the former Porto coach told reporters. "It would really complete a good group run. It would be good to finish on 18 points. We are qualified and Anzhi are as well. They go on a two-month break now for their winter break, so all of this adds to special circumstances in a game.
"But we will try to motivate our players to focus on a win and achieve 18 points and go through the game without any injuries."
Tottenham won the previous meeting 2-0 in Russia thanks to goals from the competition's joint-top scorer, Jermain Defoe, and Nacer Chadli.