Marcello Lippi has settled on a starting XI for the clash against Paraguay and Antonio Di Natale will have to be content with a place on the bench.
The former Juventus boss has opted to include Genoa defender Domenico Criscito and Riccardo Montolivo in the starting line-up, while Antonio Di Natale may start from the bench.
Di Natale was Serie A's top scorer in 2009-10 with 29 goals.
The Azzurri will begin their World Cup defence with a 4-2-3-1 formation, a system Lippi has been testing ahead of the tournament in training and friendlies, but with mixed results as the team lost to Mexico and drew Switzerland in underwhelming performances.
Meanwhile, veteran Gianluca Zambrotta should edge out Napoli's Christian Maggio on the right and Fiorentina star Alberto Gilardino will lead the line in attack.
Probable Italy line-up (4-2-3-1): Buffon; Zambrotta, Cannavaro, Chiellini, Criscito; De Rossi, Montolivo; Iaquinta, Marchisio, Pepe; Gilardino.
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