The defensive midfielder will miss the remainder of the tournament after injuring his groin against Mexico.
The AC Milan midfielder was withdrawn from his side's last 16 clash with Mexico with less than 10 minutes played in Fortaleza on Monday, after which coach Louis Van Gaal said the 29-year-old's tournament could be over.
The Royal Dutch Football Association on Tuesday confirmed he would play no further part in Brazil and could be sidelined for up to a month.
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"An MRI examination at the hospital confirmed that Nigel de Jong has suffered with a tear in his groin muscle. The 75-times capped international is therefore ruled out for two-to-four weeks,” read a statement on the national team website. "De Jong, who last Sunday in the last 16 match against Mexico was injured after nine minutes, will remain with the team in Brazil to work on his rehabilitation."
The Netherlands continues its bid for a first World Cup crown when it travels to Salvador on Saturday for a quarterfinal clash with Costa Rica.