World football's governing body revealed that over 85 ticket-less Chile fans were arrested after breaking into the stadium's media center and forcibly trying to access the stands
Dozens of supporters broke through a security gate and smashed through the doors to the center before pulling down a temporary partition wall in a bid to reach the stands.
Stewards and security officials eventually pinned down the group in a corridor next to the media center and held them there for a short time before leading them to police.
FIFA insists none of the supporters managed to reach the stands and says such "violent" acts will not be tolerated.
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"Ahead of the Spain-Chile match at the Maracana, a group of individuals without tickets violently forced entry into the stadium," a statement issued on Twitter read.
"They broke fences and overran security before being contained by security. They did not make it to the seats.
"The situation was brought under control quickly and at least 85 intruders were detained by the military police of Rio.
"The organizers of the World Cup condemn these acts of violence. We’ll communicate further info and measures to be taken in due course."
This latest incident constitutes the second security breach at the Maracana inside a week and raises serious questions about the capability of FIFA and Rio authorities to maintain safety at next month's World Cup final.
On Sunday around 30 ticket-less Argentina supporters scaled an outer wall and broke through a gate before being detained prior to their team's Group F opener with Bosnia-Herzegovina.