Jorge Sampaoli has started to bring his roster down, beginning with a pair of forwards in their 30s and one young defender.
Chile has cut Esteban Paredes, Gustavo Canales and Enzo Andia from their initial 30-man FIFA World Cup squad.
Coach Jorge Sampaoli announced his preliminary squad on Tuesday with the knowledge that a total of seven players would end up disappointed before his final selection is confirmed on June 2.
Paredes, 33, appeared at the 2010 World Cup in South Africa and has played for his country 33 times since making his debut in 2006, scoring nine goals in the process.
The attacker was also in sensational form for club side Colo Colo during this year's Clausura stage of the Chilean Primera Division, netting 16 times in 13 games.
Fellow forward Canales had previously only played a total of 18 minutes for his national team, with that appearance coming in heavy 4-0 World Cup qualifier defeat to Uruguay on November 11, 2011.
Like Paredes, 32-year-old Canales has enjoyed a good campaign at club level, scoring 12 times in 20 Primera matches for Union Espanola.
Andia, a 21-year-old center back with four caps who found himself linked with Bayer Leverkusen in 2013, will also not be on the team's plane to Brazil.
The Chilean Football Federation released a brief statement on Saturday thanking the players on behalf of the team's coaching staff for their "commitment" since earning their initial call-ups.
Chile's Group B campaign gets underway against Australia on June 13, before matches against reigning champions Spain and 2010 runner up the Netherlands.