Several members of the Roja squad hoped to commemorate the Real Betis defender, who passed away after losing his battle with cancer on Monday at the age of 23.
The Real Betis defender lost his battle with pelvic cancer earlier this week, aged 23, and La Roja stars Cesc Fabregas and Sergio Ramos led the tributes to the former Under-19 international.
Spain's players had hoped that Roque's passing would be formally recognized just before kickoff in their meeting with the Seleccao in Donetsk but the European football governing body has decided against doing so.
UEFA did not allow a minute's silence for Thierry Ronald ahead of France's clash with Sweden during the group stages either, even though the French journalist was a close friend of president Michel Platini.
Roque, who spent four years at Liverpool, was diagnosed with cancer last year after undergoing a routine checkup on a back problem and had a tumor removed last May.