The center-half has yet to fully recover from a hamstring injury sustained in the warm-up defeat by Bulgaria and will not be risked by the Oranje in Kharkiv
The 32-year-old center-half pulled up with a hamstring problem during his side's recent 2-1 friendly defeat to Bulgaria, but the Oranje boss was initially upbeat on the Malaga man's chances of being fit for the Group B clash in Kharkiv.
However, van Marwijk revealed on Wednesday evening that Mathijsen will not be risked against the Danes and that he is even considering drafting in a replacement.
"There's still a chance that Joris will recover in time for the match against Germany," the 60-year-old coach explained to reporters. "He's not going to make Saturday's game, though. I can basically rule that out. Mathijsen needs more time to recover than we were hoping for.
"We will decide in the next two days whether he stays with the group, or whether we will call up a replacement."
Mathijsen's unavailability means that either Ron Vlaar or Wilfred Bouma is likely to start against Denmark.
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