The Uruguayan came on to play the final 15 minutes as the Blaugrana beat Leon 6-0 on Monday, but didn't feature alongside the Argentine on his Camp Nou bow.
Barca boss Luis Enrique revealed on Sunday that Suarez would play a part in the game, but the Uruguayan had to wait until the 75th minute before he was introduced to an expectant Camp Nou crowd.
Messi, who received the Man of the Match award after scoring the first goal and setting up another, had already left the pitch by then as he was withdrawn among a cluster of changes with 65 minutes on the clock.
Suarez, who was presented to the fans before kickoff following his 88 million euro move from Liverpool, was made to wait a little longer but finally came on with 15 minutes left.
The Uruguayan found himself celebrating a goal before he had even touched the ball, but eventually linked up well with his teammates around the box as Barca finished with a flourish.
The 27-year-old was able to make his Barca bow in the friendly after the CAS (Court of Arbitration for Sport) deemed on Thursday that he can take part in football-related activities again following his initial ban for biting Italy's Giorgio Chiellini in the World Cup.
The four-month ban in competitive fixtures still stands, however, and Barca fans will now have to wait until Oct. 25 for a mouthwatering meeting with Real Madrid at the Santiago Bernabeu in which Suarez should make his full debut for the Catalan club and link up with Messi (and Neymar - taken off at halftime after his two goals on Monday) for the first time.
The Uruguayan, who has been training with his teammates since Friday, will be unveiled to the media on Tuesday alongside sporting director Andoni Zubizarreta.