Mourinho was charged with improper conduct after entering the field of play to approach match referee Chris Foy in the 90th minute of the loss at Villa Park.
The Chelsea boss denied the charge and requested a personal hearing, but the ruling was upheld on Wednesday. Mourinho was also warned with regards to his future conduct.
Foy sent off Willian for two bookable offences during the defeat, before Ramires was shown a straight red card for a foul on Karim El Ahmadi.
Mourinho was eventually sent to the stands by Foy and after the game suggested he should not be sent to referee Chelsea games in the future.
"Maybe it's helpful that the [referee's] committee doesn't send him [Foy] to our matches," Mourinho told reporters. "I don't have the right to request [that]. It's just I think they have to analyze the situation and see if every time he has Chelsea - or not every time - but many times he has Chelsea and problems are there, I think maybe it would be a good decision."