The midfielder could have been forced to miss the Bianconeri's encounter with Livorno after missing Chile's match against Brazil with injury, but FIFA has permitted him to play.
The 26-year-old Chile international missed his country's encounters with England last Friday and Brazil on Tuesday, and as a result could have been declared ineligible to play for the Bianconeri this weekend because of the 'five-day rule', which states that a player cannot feature for his club for a minimum of five days if he missed international duty through injury.
Juve asked the Chile FA for special dispensation that would let Vidal play on Sunday but it refused, forcing the club to go straight to FIFA to request permission to play the midfielder.
FIFA has granted the request, which is a boost to Antonio Conte's men who are contemplating playing Vidal in defense as their backline is ravaged by injuries and suspensions.
Center backs Angelo Ogbonna and Leonardo Bonucci are suspended for the trip to Tuscany following the former's red card and the latter's fourth booking of the Serie A campaign against Napoli.
Stephan Lichtsteiner is out and Andrea Barzagli is struggling to be fit in time for the clash as he has muscular problems, which means Vidal could slot in beside Martin Caceres and Giorgio Chiellini, who has recovered from injury.
Juventus trail league leaders Roma by one point and is currently on a four-game winning run.