Gerardo Martino's men, without the injured Lionel Messi, will want to avoid falling to defeat against the SPL champion for a second successive season.
Barcelona will be without Lionel Messi as the club seeks to avoid another upset against Celtic in the Champions League on Tuesday.
Last November, Neil Lennon's men sprung a huge shock at Celtic Park when goals from Victor Wanyama and rookie striker Tony Watt secured a famous 2-1 triumph over the four-time European champion.
Messi pulled a late goal back for Barca that night but the Argentinian is set for at least two weeks on the sidelines after injuring his hamstring in Saturday's 2-0 victory at Almeria.
His absence is a major blow for the Catalans, with Messi having helped himself to a hat trick as Barca brushed Ajax aside 4-0 in the Group H opener a fortnight ago to move ahead of Mario Gomez and Filippo Inzaghi by becoming the first player to score four trebles in the competition.
However, coach Gerardo Martino has more than enough in reserve to ensure that Barca will still be the favorite.
Spain international Pedro matched his illustrious teammate's efforts when he took home the match ball as Rayo Vallecano was dispatched 4-0 a week ago.
Messi scored again on Saturday as the Catalan club claimed a seventh win from as many Liga matches this term against Almeria, but was then withdrawn.
Meanwhile, Celtic warmed up for the clash in the best possible manner on Saturday when the club bounced back from a shock defeat to Morton in the Scottish League Cup with victory at Kilmarnock.
The Scottish champion survived a fightback from the home side before prevailing 5-2 as Georgios Samaras fired in a hat trick.
Barcelona will once again be without left back Jordi Alba due to a hamstring problem, while fellow defender Carles Puyol and playmaker Isaac Cuenca are recovering from knee surgery.
Celtic is missing close-season arrival Steven Mouyokolo, who ruptured his Achilles in training before the Milan clash.