The 44-year-old has been missing since the turn of the year, when he went to New York City to undergo cancer treatment, but is set to come back to Catalunya shortly.
"We are eager for him to return. He is a cornerstone for us and at this time we have noticed his absence," the Spain star told reporters.
Assistant coach Jordi Roura has been covering for Vilanova, and Busquets feels the 45-year-old has performed admirably.
"We cannot ask for more from Roura, he has shown his face in press conferences in front of us, did what he had to do and he has our appreciation," the midfielder said.