Vicente del Bosque's men lead the way ahead of Germany and Argentina in an unchanged top three, but Iberian neighbor Portugal - whom Cristiano Ronaldo fired to the World Cup late last year - has leapfrogged Colombia into fourth.
Switzerland is up two places to sixth despite not playing a game since November, while Confederation Cup holder Brazil has risen to ninth, overtaking Netherlands.
Denmark is among the biggest movers, jumping from 25th to 20th, and France is up two spots to joint 18th with Ukraine, whom Didier Deschamps' side beat in a playoff in November to qualify for the World Cup.
World Cup debutant Bosnia & Herzegovina also rises two places to 17th, with United States and Chile up one spot each to 13th and 14th at the expense of England, which falls to 15th.
Mexico has held steady at 21st, after qualifying for the World Cup with a comprehensive playoff win over New Zealand.
Cote d'Ivoire has dropped out of the top 20 after a six-place fall, but African rival Cape Verde is up eight places to 27.
Mali is one of the list's biggest losers, dropping 19 places to 59th, while Ghana - which will look to replicate its run to the 2010 World Cup quarterfinals in Brazil later this year - has plummeted 13 spots to number 37.
Elsewhere, Armenia has moved inside the top 30, its highest ever FIFA ranking, after a World Cup qualification campaign that saw the country narrowly miss out on a playoff spot.